RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance

RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance - List of Reported Hits by Jianwu Bai

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Final Hit	HFA Amplicon	DRSC Amplicon	"S" Primer	"R" Primer	Amp. Length	Gene	19 bp Matches	CAR
2	HFA08450	DRSC08450	AACTTCGCTGTCGTAAGAG	CAGACGGATCTGCATCCC	519	Fit1	1	No
3	HFA09662	DRSC09662	CTATTTGATCGAATCGTCCAA	ACGTCCAGCGGCACAC	132	Ac76E	0	No
3	HFA10471	DRSC10471	TCCAAATTGGAATTTGTCATGATA	TCTGGGCCTCCAGTTCAG	519	CG5027	0	No
3	HFA10559	DRSC10559	TGTGGCTCTAGATCGTAAAG	GCCTCTCACTATTACCTAACC	515	CG5931	0	No
3	HFA10681	DRSC10681	GATCGCAACGACATAAGTGA	CAATGGTCTGGTATTTGCCC	517	CG6640	1	No
2	HFA10779	DRSC10779	GTCCGACTGCACATCACC	TTCCGTGAGGCAATCATCT	510	Idh	0	No
2	HFA10908	DRSC10908	GCAGATCCCGATAGGTGAA	GAAGGCTCGTCTGAATGTG	500	Fit2	0	No
3	HFA11046	DRSC11046	CTGCGATTTCCTTCTCCTT	CGAGAAGGTGGACATTTGG	512	wnd	0	No
3	HFA11255	DRSC11255	TTCGACTTCCAAGGACTTCT	CACCGAGCTGGAGATGTC	502	Prm	1	No
3	HFA11256	DRSC11256	CAGCGGCAGAGATGAAG	CCATCGCCGGAGATGAC	516	Pros26	0	No
3	HFA11257	DRSC11257	TGTGCAGCACGGTTGAG	CTGTGGAGCTGGAACCG	512	Prosbeta2	0	No
3	HFA11270	DRSC11270	CTGCCTGAGATGGTCCTTA	TGTTCTGTGCATCCTAGCC	250	CG17672, RpS12	0	No
3	HFA11273	DRSC11273	AGACGCTTCTCGTCCTTC	GTGAACGGCCGCATACC	212	RpS9	0	No
3	HFA11274	DRSC11274	AATCGTCTGCAGCAAGATC	GTTTTGTTAACGCCGGTTTC	506	Rpn1	0	No
3	HFA11297	DRSC11297	GGCGTCGCGTTTCTTTT	ATTGTGCAAATACGTGGACC	501	Taf4	1	No
3	HFA11298	DRSC11298	AAATCCATATCCACTTCCTCAC	AAGGAAAGCGCATTTCGT	501	Taf2	2	No
3	HFA11309	DRSC11309	CGGAAAACATTCGCCAATAC	CGTGCTTGCCCCTTATCT	318	Trn	4	No
3	HFA11342	DRSC11342	TCTTCTTGAATAGCGAGTAGT	AGCGCCCCCAGCACC	361	eIF-4E	1	No
2	HFA11384	DRSC11384	TTCCGTTGATAAGATTAGTGAG	TCCTTCGAGGTGGACATCT	505	polo	2	No
2	HFA11609	DRSC11609	TGGTTTTGGCGATCCTGTT	AAGCCCCAAGTGATAGATGA	497	rgn	0	No
2	HFA11791	DRSC11791	CCTCTCCGCTCCGTTG	AGTGGCGTGGTTGGAGTT	432	CG6020	1	No
3	HFA12127	DRSC12127	ATTCGCTTCTGTTGGTACTG	CGCAAGCACGGAAAGAA	489	CG9776	0	No
3	HFA12234	DRSC12234	CCTGCGGCTGTTCGTT	GGATTGGACAGCGGTCTG	517	growl	0	No
2	HFA12310	DRSC12310	TGGTGTTGTTGGGTTGCTG	ATCAAAGCCATCGAGAAGAC	511	atms	1	No
3	HFA12332	DRSC12332	CGCAGCAGACGCAGAC	GGCAACCAGCTGGATCG	568	CG9779	2	No
3	HFA12337	DRSC12337	CCCCAGTATTGCAACCGTT	CCATTTTCACCCACCAGTTC	258	CG31523	1	No
3	HFA12372	DRSC12372	CGACCCACATTTATTGTGACA	CTGGAAAAACGCTTGTCTTC	514	abs	0	No
3	HFA14314	DRSC14314	TCACTCACCTCAGTGATCTT	ATGTCGGACATTGAGGATGT	230	tmod	0	No
3	HFA14322	DRSC14322	GGGCCGGCGCTGTTT	CAGCAACAACAACAACCTCC	512	pygo	8	No
3	HFA14925	DRSC14925	CTTCGGTGGCCATCTTTTT	AAGAAGACGATAGAGCCTGT	496	CG31374	3	No
2	HFA15036	DRSC15036	GAAGCAGTTCAGATCAATAAAG	CCTGTGGCCATGGGAAAAA	405	CG1544	0	No
3	HFA15395	DRSC15395	AGTTCAGCTTTGGCATCTTT	CCGGCAACTGGTGATTAAAT	361	CG1890	1	No
3	HFA15409	DRSC15409	CGGTGATGACGTTGATGTC	CGTTTGAGCTCGACTTGG	498	RhoGAP100F	0	No
3	HFA16113	DRSC16113	TGCGTTCGAGCAACTGA	CAGCGAGGCGCCTAG	494	CG31374	3	No
3	HFA16741	DRSC16741	CTCCTCCTCCTCCTCTTCA	ACTCATGGATGCCGACAAG	406	Mlc2	1	No
2	HFA16758	DRSC16758	ATGGCGGGATTGATGAACC	CTTTGGCTAATGTGGTGACTA	517	Nf1	2	No
3	HFA16833	DRSC16833	CCTTGGCCAGCACCTTG	CGCAGCGCTTCCGTG	507	RpL4	3	No
3	HFA16839	DRSC16839	CGAGCGGCGCAACAC	GGAGTAATTCATCGGGGTCA	519	Rpn2	0	No
3	HFA17062	DRSC17062	ACATTTGTTAGCGAGAGGAC	GCTGATCGAGCACATCAAT	582	tmod	1	No
3	HFA17129	DRSC17129	CTTGCTGAGCTAAAAGTTAATC	CGTTCCTGTTATAAAGGATAAG	516	bt	0	No
3	HFA17154	DRSC17154	CTCCATTGCTATCACGAATAC	TGCTTTTATCTATGGACATGAG	440	CG42314	0	No
3	HFA17171	DRSC17171	CTCCAAGTCCACAAAATGTTTA	GTAAAAATTTCCAATTTTGATGACC	501	bt	1	No
3	HFA17724	DRSC17724	CTGACGGCGGAGCATTT	CTGGAGTACCATGACTGTGT	513	Actn	1	No
3	HFA18184	DRSC18184	GCCGAGTTGGCAGACTAT	CAAAGCGGGCGAACTTTA	505	crn	3	No
3	HFA18403	DRSC18403	GGCCAGCAGAGACTCGT	ACAAGCGGAATCCCTCCA	505	lva	0	No
3	HFA18427	DRSC18427	GACAGATTCGATATCCTAATGG	GGTGGAGAACGGACAGAAG	504	CG8636	1	No
3	HFA18509	DRSC18509	CACCTCTTGCAGTTGGTCC	TCAAGCTGTCCGATCTCAA	515	CG3457	1	No
3	HFA18728	DRSC18728	GCGACCAGCGCTGATAA	CTTCAGCACCCGGAAATC	513	Vinc	0	No
3	HFA18755	DRSC18755	GCTCAGATGCAATAGCTGTA	TAGCCCTAGGCGAAACAG	495	crn	0	No
3	HFA18760	DRSC18760	CCGCCTTGGATTTGGTGT	TCCCGAGTACAGCCACT	407	deltaCOP	1	No

Select Columns To Display:
Assay Plate Information
Details ?
Results Information
Phenotype
Final Hit ?
Amplicon Information
HFA Amplicon ID ?
DRSC Amplicon ID ?
"S" Primer ?
"R" Primer ?
Amplicon Length(s) ?
Hits in Public Screens ?
Chromosomal Location Information
Number of Chromosome Locations ?
Chromosome Locations ?
Chromosome ?
Chromosome Start ?
Chromosome Stop ?
Gene Information
Gene Symbol ?
Flybase Gene ID ?
Deprecated Flybase Gene IDs ?
Gene Name ?
Gene Synonyms ?
NCBI Nucleotide Accessions ?
Entrez Gene / LocusLink Identifiers ?
UniGene Identifiers ?
RefSeq RNA Accessions ?
HomoloGene Group Identifiers ?
Homo sapiens Gene(s) via Homologene ?
Mus musculus Gene(s) via Homologene ?
Caenorhabditis elegans Gene(s) via Homologene ?
Saccharomyces cerevisiae Gene(s) via Homologene ?
Homo sapiens Putative Orthologues According to InParanoid ?
Mus musculus Putative Orthologues According to InParanoid ?
Caenorhabditis elegans Putative Orthologues According to InParanoid ?
Saccharomyces cerevisiae Putative Orthologues According to InParanoid ?
Misc. Organism Putative Orthologues According to InParanoid ?
Homophila - Possible Human Disease Homologs ?
Gene Ontology ?
Protein Domains ?
Potential Off-Target Effect Information
Number of Potential Off-Targets Based On 17 bp Matches ?
Number of Potential Off-Targets Based On 19 bp Matches ?
Number of Potential Off-Targets Based On 21 bp Matches ?
Number of Potential Off-Targets Based On 23 bp Matches ?
Number of Potential Off-Targets Based On 25 bp Matches ?
CAR Repeats ?
CAN Repeats ?

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