The TRiP stocks are in a y v; attP2 genetic background (genotype: y[1] v[1]; P{y[+t7.7]=CaryP}attP2).

All TRiP stocks have the genotype:
y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JFXXXXX}attP2 or
y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMXXXXX}attP2, where JF are TRiP stocks made during our pilot project at Janelia Farm and HM are TRiP stocks made at Harvard Medical School.

The attP docking site of all TRiP stocks is attP2 located on the third chromosome at 68A4.

All TRiP stocks have a wildtype appearance as the wildtype vermillion gene is used as the selection marker for the hairpin transformants and the attP insertions carry the wildtype yellow gene.

No TRiP stocks contain "mini-white".

All TRiP stocks have the genotype:
y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.JFXXXXX}attP2 or
y[1] v[1]; P{y[+t7.7] v[+t1.8]=TRiP.HMXXXXX}attP2, where JF are TRiP stocks made during our pilot program at Janelia Farm and HM are TRiP stocks made at Harvard Medical School.

Many of the TRiP lines have been tested for their "knock-down" abilities. Since this initial testing, however, the TRiP has entered the "production phase" where not all lines are being tested.

The GAL4 drivers commonly used by the TRiP are listed in the TRiP Toolbox. Some of these drivers also contain UAS-dicer2, which enhances the knock-down. These lines can be obtained from the BDSC in Fall 2008.

If the TRiP stock you are using was constructed in the VALIUM10 vector, it is likely that UAS-dicer2 will not be necessary to achieve maximum knock-down of your target gene.

However, if the TRiP stock you are using was construction in the VALIUM1 vector, it is essential that UAS-dicer2 be carried in the GAL4 driver you are using.

To determine which VALIUM vector your TRiP stock utilized please refer to the list of available TRiP stocks.